Document Details

Document Type : Article In Journal 
Document Title :
Glomerular changes in microscopic haematuria, studied by quantitative iminunoelectron microscopy and in situ zyinography
يتغيّر جلوميرولار في الهيماتيوريا الدّقيق، مدروس باستخدام المجهر إيميننويليكترون كمّيّ و بالموضع الأصليّ زايينوجرافي
 
Subject : Glomerular changes in microscopic haematuria, studied by quantitative iminunoelectron microscopy and in situ zyinography 
Document Language : English 
Abstract : Background. Hacrnaturia of glomerular origin, even if mild, implies the development of defects in th glomerular basement membrane (GBM). In diseases where there is no infiltration of leukocytes into the glomerulus——such as thin basement membrane disease (TBMD) and histologically mild cases of IgA ncphrop athy (IgAN)—the mechanism by which such defects form is unclear. Methods. Frozen renal tissue from 18 cases of TBMD, 18 of mild IgAN and 18 cases with no detectable abnormality were studied: (I) by quantitative in suit zyrnography, to estimate the activity of glomerular collagenases; and (ii) by quantitative immunoelectron microscopy to estimate the amount of major basement membrane proteins per unit length and per unit area of glomerular basement membrane. Results. Cases of IgAN showed considerably more glornerular collagenase activity than normal (P=O.OOI). Thin basement membrane disease showed no difference in collagenase activity. A count of LCAp ositive cells in glomeruli confirmed that the IgAN cases did not show glomerular leukocyte Infiltration. Conversely, cases of IgAN showed no difference in (iBM composition from normal, nor was any differe nce in GBM thickness detected in this group. However, cases of TBMD showed considerably less laminin (P=O.0008), fibronectin (P=O.002) and type VI collagen (P=O.0005) per unit length of basement membrane. Collagen IV showed a smaller reduction per unit length (P=O.Ol), but unlike the other protein studies it appeared to be present in higher concentrat ion per unit area (P=O.03), suggesting that it is more ‘compact’ in TBMD disease. Conclusions. Two distinct mechanisms of haernaturia seem to be involved in these two conditions. In IgAN there is increased activity of enzymes that can degrade 
ISSN : 0931-0509 
Journal Name : Nephrology Dialysis Transplantation 
Volume : 17 
Issue Number : 1 
Publishing Year : 1421 AH
2002 AD 
Article Type : Article 
Added Date : Saturday, March 23, 2013 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ابراهيم حسن الزهرانيalzhrani, ibrahim hassanResearcherDoctorateibrahimzahrani@hotmail.com

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