Document Details
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Document Type |
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Article In Journal |
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Document Title |
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Assess the genotoxicity of leaf extract Rue using mutations want test food in Saccharomyces cerevisiae... تقييم السمية الوراثية لمستخلص اوراق نبات الحرمل باستخدام اختبار طفرات العوز الغذائي في خميرة الخباز |
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Subject |
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Assess the genotoxicity of leaf extract Rue using mutations want test food in Saccharomyces cerevisiae... |
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Document Language |
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English |
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Abstract |
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In this study, assessing the genotoxicity of a medicinal plants used in folk medicine in Saudi Arabia, a plant Harmal Razi through search capability aqueous extract of leaves on the development of mutation chromosomal and mutation gene and activity Altsrtunai on the device genetic and using several biological systems as follows: 1 - cells of Vicia roots plant onions were detected imbalances الكروموزومية in test onions after treatment cells of Vicia roots in three concentrations of Rue periods of time varying, results showed a decline significantly in function split with Motta collective cells compared experimentally control, especially when high concentrations and periods longer exposure, in addition to the high proportion of interphase morally at the expense of the rest of the phases of the split with some recording imbalances الكروموزومية and that was most notably tropical Alkulchesina, which indicates that the extract Rue role in carcinogenesis and influential resistance toxic cellular organisms. Also been isolated and purified DNA of all transactions, including the control treatment, results were recorded a significant decline in the amount of DNA in all transactions compared to the control experiment. Also been detected molecularly for mutations technology multiplier random pieces varying from DNA (RAPD) for all transactions, has shown pictures gel variation in style Hazmi between transactions and experiment control, which indicates Balmekdrh Alotforia of Harmal, then was isolated and study total protein of all transactions where observed significant decrease in the amount of protein as compared to the control, and electrically separate the total protein-acrylamide gel, the disparity in the results were recorded numbers and weights of packages separated electrically as compared to the control, which confirms the estimated Alotforia Harmal. 2 - germs Alkonidah fungus أسبرجيللس gear has been detected Auxotrophic mutants of the fungus after treatment with four concentrations of Rue and five periods for each concentration, and if possible to isolate 14 boom Oz diet, they all need to amino acids, has varied needs careful of amino acids in many of mutations, also were subjected to DNA and protein mutations and wild type for all molecular analyzes that underwent plant onions. Where results were similar to the previously in terms of the amount of DNA, protein and Hazmi pattern for them, which confirms the behavior Alotfora extract. 3 - lymphoid blood cells to humans has been detected nuclei minute and many imbalances الكروموزومية in human lymphocytes after treatment with three concentrations of Rue for three consecutive days has been registered all Alcetologih changes already observed in test onions. Which confirms that the extract Rue Mtafra Kromozumia and supports its potential role in the resistance carcinogenesis, and has been detected defect caused to DNA per cell on alone through the application of test comet Comet assay, results showed a rise significantly in the number of cells caudate compared experimentally control, which confirms to extract Rue material mutagenic |
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ISSN |
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6360-1110 |
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Journal Name |
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Egyptian Journal of natural toxins... |
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Volume |
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3 |
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Issue Number |
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1 |
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Publishing Year |
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1425 AH
2005 AD |
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Article Type |
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Article |
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Added Date |
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Wednesday, February 20, 2013 |
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Researchers
| ندى حسن التواتي | Touati, Nada Hassan | Researcher | Doctorate | naltwaty@yahoo.com |
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