Document Details

Document Type : Article In Journal 
Document Title :
Ultrastructural immuno gold study on the various cell types of cultured pancreatic islets of adult rats
دراسة الذهب التركيبية المناعية على مختلف أنواع الخلايا من الجزر البنكرياسية مثقف من الفئران الكبار
 
Subject : Ultrastructural immuno gold study on the various cell types of cultured pancreatic islets of adult rats 
Document Language : English 
Abstract : Whereas several reports describing the ultrastructure of the intact pancreatic islets have been recorded, published experience with the ultrastructural integrity of the cultured pancreatic islets is limited. The present study was, therefore, undertaken to provide an ultrastructure identification of the different cells in the cultured islets of the adult rat pancreas, after marking their secretory granules with gold particles. Pancreatic islets were isolated from adult male Wistar rats by the intraductal perfusion of collagenase technique. The islets were cultured in RPMI-1640 medium for 3 days and processed for preparation of ultrathin sections. The sections were stained with the indirect immunogold technique for insulin, glucagon, somatostatin, and pancreatic polypeptide. Ultrastructural examination of the cultured islets clearly identified the presence of B, A, D and PP-cells, as indicated by the numerous gold particles concentrated predominantly over the secretory granules. The secretory granules of the various cell types of the cultured islets demonstrated several similarities as well as differences from the recorded results of the corresponding secretory granules of the intact islets. The differences probably reflect a deviation in the underlying mechanisms of synthesis, maturation and secretion of the different secretory products of the cells in the cultured islets as they adapt to the in vitro environment. 
ISSN : 0015-5659 
Journal Name : Folia Morphol 
Volume : 59 
Issue Number : 4 
Publishing Year : 1420 AH
2000 AD 
Article Type : Article 
Added Date : Saturday, February 19, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
مصطفى النجارalnajar, mustafa InvestigatorDoctorate 

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