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Document Type |
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Article In Journal |
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Document Title |
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Morphogenesis of Two Candida albicans Strains as Influenced by Growth Media, pH Value and Incubation Temperature التّطوّر البنائيّ لتوتّري البيكانز المبيضة كما هو متأثّر بإعلام النّموّ، مقياس الحموضة و حرارة فترة الحضانة |
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Subject |
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Morphogenesis of Two Candida albicans Strains as Influenced by Growth Media, pH Value and Incubation Temperature |
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Document Language |
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English |
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Abstract |
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The pattern of growth and mycelial formation of two strains M (oral isolate) and G (genitourinary tract isolate) of Candida albicans, in horse-serum, MSB and RP1v.II 1640 media, indicated that the first medium promotes filamentation in both strains. While, MSB intiates mediun mycelial formation for M strain and RP1v1I stimulates the yeast growth and low filamentation. However, RP1v.1I promotes moderate filamentation and growth values for C. albicans G. The peak of mycelium production appeared between 1.5 and 4.5 h after inoculation of C. albicans M and between 1.5 to 6.0 and 7.5 h for C. albicans G. The pH value of 7.4 appeared to be optimal for filarnentation on MSB at 37CC for C. albicans, M (oral pH slightly alkaline), While, pH of 4.4 was responsible for highest mycelial formation of C. albicans G, (vaginal pH around 4.5) under same cultural conditions. Incubation temperature of 37°C was concomitant with the highest germ-tube formation by C. albicans M, on MSB at pH 7.4. While 34CC was optimum for filarnentation by C. albicans G, on MSB at pH 4.4. These results indicated that the pattern of filamentation by C. albicans depends mainly on the yeast strain and the nutritional and cultural conditions control the route of yeast dimorphism. The two C. albicans strains (M and G) showed the same phenotypic switching when grown in solid media of MSB, RP1.1I, horse serum and blood agar base, in presence of7% CO2 (anaerobic) and in absence of 7% CO2 (aerobic) conditions). However, C. albicans G showed phenotypic switching on chocolate agar (feet appendages in presence of 7% CO2 and normal growth in absence of CO2), but C. albicans M showed no switching under the same conditions |
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ISSN |
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1012-1319 |
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Journal Name |
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Science Journal |
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Volume |
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1 |
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Issue Number |
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2 |
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Publishing Year |
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1426 AH
2006 AD |
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Article Type |
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Article |
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Added Date |
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Sunday, February 6, 2011 |
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Researchers
| صالح عبدالله كابلي | kabli, saleh abdullah | Researcher | Doctorate | |
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